The secondary structure and architecture of human elastin

Eur J Biochem. 1998 Dec 1;258(2):533-9. doi: 10.1046/j.1432-1327.1998.2580533.x.

Abstract

The presented work constitutes the first structural characterization of both insoluble human elastin and its solubilized form, kappa-elastin. Structural data were reached following the use of Fourier transform infrared, near infrared Fourier transform Raman and circular dichroism optical spectroscopic methods and their quantitative analysis permitted us to estimate approximately 10% alpha-helices, approximately 35% beta-strands and approximately 55% undefined conformations in the global secondary structure of insoluble human elastin in the solid state. Following the use of the LINK method, the probable local distribution of the secondary-structure elements along the sequence was determined and compared to that obtained for bovine elastin, the historical standard of elastin. This comparison led us to propose a globular architecture for the human elastomer and permitted us to delineate some elements of its structure-elasticity relationship.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Circular Dichroism
  • Elastin / chemistry*
  • Humans
  • Molecular Sequence Data
  • Protein Structure, Secondary*
  • Sequence Alignment
  • Solubility
  • Spectroscopy, Fourier Transform Infrared
  • Structure-Activity Relationship

Substances

  • Elastin