Target specificity of immunoglobulin class switch recombination is not determined by nucleotide sequences of S regions

Immunity. 1998 Dec;9(6):849-58. doi: 10.1016/s1074-7613(00)80650-0.

Abstract

We describe a model system for class switch recombination (CSR) using CH12F3-2 cells transfected with a DNA construct containing two S sequences transcribed by different promoters and separated by a viral thymidine kinase (TK) gene. Recombination observed using this system shares key properties with physiological CSR: deletion of DNA between two S regions, requirement for cytokine stimulation, and nonhomologous and no consensus breakpoint sequences. Studies on transfectants with variants of this construct led us to the following conclusions: (1) two S sequences are required for CSR; (2) isotype specificity of recombination is not determined by nucleotide sequences of S regions; (3) S sequences are not strand-specific; and (4) induction of recombination activity requires cytokine stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CD40 Ligand
  • Cell Line
  • Drug Resistance
  • Ganciclovir / pharmacology
  • Immunoglobulin Class Switching / genetics*
  • Immunoglobulin Switch Region / genetics*
  • Immunoglobulin Switch Region / immunology
  • Immunoglobulin alpha-Chains / genetics*
  • Immunoglobulin mu-Chains / genetics*
  • Interleukin-4 / pharmacology
  • Membrane Glycoproteins / pharmacology
  • Mice
  • Molecular Sequence Data
  • Recombination, Genetic
  • Transforming Growth Factor beta / pharmacology
  • Tumor Cells, Cultured

Substances

  • Immunoglobulin alpha-Chains
  • Immunoglobulin mu-Chains
  • Membrane Glycoproteins
  • Transforming Growth Factor beta
  • CD40 Ligand
  • Interleukin-4
  • Ganciclovir