IS990, a new species-specific insertion-sequence-related element of the Mycobacterium tuberculosis complex

J Dziadek; A Sajduda; J W Dale; J J McFadden

doi:10.1099/00221287-144-12-3407

IS990, a new species-specific insertion-sequence-related element of the Mycobacterium tuberculosis complex

Microbiology (Reading). 1998 Dec:144 ( Pt 12):3407-3412. doi: 10.1099/00221287-144-12-3407.

Authors

J Dziadek¹, A Sajduda², J W Dale³, J J McFadden³

Affiliations

¹ Centre for Microbiology and Virology, Polish Academy of Sciences, Lodowa 106, 93-232 Ldz, Poland.
² Department of Genetics of Microorganisms, University of Ldz, Banacha 12/16, 93237 Ldz, Poland.
³ Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK.

PMID: 9884233
DOI: 10.1099/00221287-144-12-3407

Abstract

The structure and distribution of 1S990, a new Mycobacterium tuberculosis DNA sequence with homology to characterized insertion sequences (ISs), were investigated. IS990 was related to IS elements of the IS3 family and was present as a single copy in all 21 investigated M. tuberculosis strains, two Mycobacterium bovis strains and two M. bovis BCG strains. The sequence appears to be specific for the M. tuberculosis complex. The element carries two frameshift mutations and appears to be defective.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
DNA Transposable Elements / genetics*
DNA, Bacterial / analysis
Gene Dosage
Molecular Sequence Data
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Species Specificity

Substances

DNA Transposable Elements
DNA, Bacterial