Abstract
Lipochitooligosaccharides (LCOs) synthesized by Azorhizobium caulinodans ORS571 are substituted at the nonreducing-terminal residue with a 6-O-carbamoyl group. LCO biosynthesis in A. caulinodans is dependent on the nodABCSUIJZnoeC operon. Until now, the role of the nodulation protein NodU in the synthesis of azorhizobial LCOs remained unclear. Based on sequence similarities and structural analysis of LCOs produced by a nodU mutant, a complemented nodU mutant, and Escherichia coli DH5 alpha expressing the nodABCSU genes, NodU was shown to be involved in the carbamoylation step.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism*
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Carbohydrate Sequence
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Carboxyl and Carbamoyl Transferases*
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Chromosome Mapping
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Escherichia coli / genetics
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Fabaceae / microbiology
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Genes, Bacterial
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Genetic Complementation Test
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Lipopolysaccharides / chemistry
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Lipopolysaccharides / metabolism
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Molecular Sequence Data
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Mutation
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Operon
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Plants, Medicinal
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Rhizobiaceae / genetics*
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Rhizobiaceae / metabolism*
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Sequence Homology, Amino Acid
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Spectrometry, Mass, Fast Atom Bombardment
Substances
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Bacterial Proteins
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Lipopolysaccharides
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lipid-linked oligosaccharides
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Carboxyl and Carbamoyl Transferases
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nodU protein, Bacteria