Complementation of the growth defect of an rnpA49 mutant of Escherichia coli by overexpression of arginine tRNA(CCG)

Biochem Mol Biol Int. 1998 Dec;46(6):1153-60. doi: 10.1080/15216549800204712.

Abstract

We previously found that overexpression of arginine tRNA(CCG) from Brevibacterium albidum complements the rnpA49 mutation, which is responsible for the thermosensitivity of Escherichia coli RNase P function. In this present work, we show that the E. coli homologue tRNA also complements the same mutation, but other tRNAs do not. These results suggest that the rnpA49 mutation causes a major cellular defect in an RNase P reaction to generate the mature arginine tRNA(CCG).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Brevibacterium / enzymology
  • Brevibacterium / genetics*
  • DNA Primers
  • Endoribonucleases / chemistry
  • Endoribonucleases / genetics*
  • Enzyme Stability
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutation*
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction
  • RNA, Catalytic / chemistry
  • RNA, Catalytic / genetics*
  • RNA, Transfer, Arg / chemistry
  • RNA, Transfer, Arg / genetics*
  • RNA, Transfer, Pro / chemistry
  • RNA, Transfer, Pro / genetics
  • Ribonuclease P
  • Thermodynamics

Substances

  • DNA Primers
  • Escherichia coli Proteins
  • RNA, Catalytic
  • RNA, Transfer, Arg
  • RNA, Transfer, Pro
  • Endoribonucleases
  • Ribonuclease P
  • ribonuclease P, E coli