Abstract
Transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6, are characterized by the presence of a single cut domain and by a peculiar homeodomain (Lannoy, V. J., Bürglin, T. R., Rousseau, G. G., and Lemaigre, F. P. (1998) J. Biol. Chem. 273, 13552-13562). We report here the identification and characterization of human OC-2, the second mammalian member of this class. The OC-2 gene is located on human chromosome 18. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. The amino acid sequence of OC-2 contains several regions of high similarity to HNF-6. The recognition properties of OC-2 for binding sites present in regulatory regions of liver-expressed genes differ from, but overlap with, those of HNF-6. Like HNF-6, OC-2 stimulates transcription of the hnf-3beta gene in transient transfection experiments, suggesting that OC-2 participates in the network of transcription factors required for liver differentiation and metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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COS Cells
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Chromosomes, Human, Pair 18*
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DNA / metabolism
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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Hepatocyte Nuclear Factor 3-beta
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Hepatocyte Nuclear Factor 6
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology*
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Humans
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Liver / chemistry
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Liver / physiology*
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Molecular Sequence Data
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / genetics
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RNA, Messenger / metabolism
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Rats
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Skin / chemistry
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / physiology*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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FOXA2 protein, human
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Foxa2 protein, rat
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Hepatocyte Nuclear Factor 6
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Homeodomain Proteins
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Nuclear Proteins
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ONECUT1 protein, human
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ONECUT2 protein, human
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Onecut1 protein, rat
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RNA, Messenger
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Trans-Activators
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Transcription Factors
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Hepatocyte Nuclear Factor 3-beta
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DNA
Associated data
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GENBANK/AA121823
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GENBANK/Y18198