Abstract
WAP-8294A, produced by Lysobacter sp., is a complex consisting of water soluble depsipeptide antibiotics. It was further purified by column chromatographies and HPLC, and 19 components were obtained. WAP-8294A2, a major component, and minor components A1, A4, Ax8, Ax9 and Ax13 were active against gram-positive bacteria, in particular, methicillin-resistant Staphylococcus aureus (MRSA) in vitro. WAP-8294A2 was highly active in vivo in mice against the systemic infection of MRSA.
MeSH terms
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Depsipeptides*
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Fermentation
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Fungi / drug effects
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Gram-Negative Bacteria / chemistry*
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Gram-Negative Bacteria / classification
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Gram-Negative Bacteria / drug effects
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Gram-Positive Bacteria / drug effects
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Inhibitory Concentration 50
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Leukemia L1210 / pathology
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Macrolides
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Magnetic Resonance Spectroscopy
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Male
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Mass Spectrometry
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Methicillin Resistance
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Mice
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Mice, Inbred ICR
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Microbial Sensitivity Tests
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / isolation & purification
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Peptides, Cyclic / pharmacology
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Staphylococcal Infections / drug therapy
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Staphylococcus aureus / drug effects*
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Tumor Cells, Cultured
Substances
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Anti-Bacterial Agents
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Depsipeptides
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Macrolides
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Peptides, Cyclic
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lotilibcin