Mutations in the human immunodeficiency virus type 1 reverse transcriptase gene observed in stavudine and didanosine strains obtained by in vitro passages

Res Virol. 1998 Nov-Dec;149(6):355-61. doi: 10.1016/s0923-2516(99)80003-x.

Abstract

We have selected a human immunodeficiency virus type 1 (HIV1) using the technique of in vitro selection to generate variants that are resistant to didanosine and/or stavudine. After serial passages of the Lai strain of HIV1 in MT-2 cells in increased concentrations of didanosine-stavudine association, 2 novel mutations in reverse transcriptase at codon 57 (Asp-->His) and at codon 98 (AIa-->Val) were observed. These mutations were associated with an 11.5-fold increase in the didanosine and a 4.5-fold increase in the stavudine 50% inhibitory concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-HIV Agents / pharmacology*
  • Didanosine / pharmacology*
  • Genes, Viral
  • HIV Reverse Transcriptase / genetics*
  • HIV-1 / drug effects
  • HIV-1 / enzymology*
  • HIV-1 / genetics
  • Humans
  • Mutagenesis*
  • Reverse Transcriptase Inhibitors / pharmacology*
  • Stavudine / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Anti-HIV Agents
  • Reverse Transcriptase Inhibitors
  • Stavudine
  • HIV Reverse Transcriptase
  • Didanosine