Abstract
Efficient gene transfer into human hematopoietic stem cells (HSCs) is an important goal in the study of the hematopoietic system as well as for gene therapy of hematopoietic disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction occurred in the absence of cytokine stimulation and resulted in transgene expression in multiple lineages of human hematopoietic cells for up to 22 weeks after transplantation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD34 / analysis*
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Bone Marrow Cells / cytology
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Cell Division
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Cell Survival
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Colony-Forming Units Assay
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Gene Expression
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Gene Transfer Techniques*
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Genetic Vectors*
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Green Fluorescent Proteins
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HIV / genetics*
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Hematopoiesis
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Hematopoietic Stem Cell Transplantation*
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Hematopoietic Stem Cells* / cytology
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Hematopoietic Stem Cells* / immunology
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Humans
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Leukemia Virus, Murine / genetics
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Luminescent Proteins / genetics
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Mice
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Mice, Inbred NOD
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Mice, SCID
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Promoter Regions, Genetic
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Transfection
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Transgenes
Substances
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Antigens, CD34
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Luminescent Proteins
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Green Fluorescent Proteins