Abstract
On the basis of differences in the potencies and intrinsic activity of 5-HT4 receptor agonists in different biological models it has been suggested that there is heterogeneity among 5-HT4 receptors. Here, we report the molecular cloning of several 5-HT4 receptor splice variants in mouse, rat, and human brain. Our data suggest that the differences in efficacy of 5-HT4 ligands on 5-HT4 receptor-mediated responses in several tissues is due to differences in coupling efficiency rather than to the presence of different 5-HT4 receptor isoforms.
MeSH terms
-
Alternative Splicing*
-
Amino Acid Sequence
-
Animals
-
Brain / metabolism
-
Chromosome Mapping
-
Chromosomes, Human, Pair 5
-
Cloning, Molecular
-
Genetic Variation*
-
Humans
-
Mice
-
Molecular Sequence Data
-
Rats
-
Receptors, Serotonin / chemistry
-
Receptors, Serotonin / genetics*
-
Receptors, Serotonin / physiology*
-
Receptors, Serotonin, 5-HT4
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / metabolism
-
Sequence Alignment
-
Sequence Homology, Amino Acid
Substances
-
Receptors, Serotonin
-
Recombinant Proteins
-
Receptors, Serotonin, 5-HT4