Abstract
Bacteriorhodopsin (BR) from Halobacterium salinarum as well as halorhodopsin (pHR) and sensory rhodopsin II (pSRII) from Natronobacterium pharaonis were functionally expressed in E. coli using the method of Shimono et al. IFEBS Lett. (1997) 420, 54-56]. The histidine tagged proteins were purified with yields up to 1.0 mg/l cell culture and characterized by ESI mass spectrometry and their photocycle. The pSRII and pHR photocycles were indistinguishable from the wild type proteins. The BR photocycle was considerably prolonged. pSOII is located in the cytoplasmic membrane and the C-terminus is oriented towards the cytoplasm as determined by immunogold labelling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Archaeal Proteins*
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Bacteriorhodopsins / biosynthesis
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Bacteriorhodopsins / isolation & purification
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Bacteriorhodopsins / metabolism*
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Carotenoids*
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Cell Membrane / chemistry
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Cell Membrane / ultrastructure
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Chromatography, Affinity
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Cytoplasm / metabolism
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Cytoplasm / ultrastructure
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli / ultrastructure
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Halobacteriaceae / metabolism
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Halorhodopsins
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Histidine / genetics
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Histidine / metabolism
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Kinetics
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Lipid Bilayers / metabolism
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Mass Spectrometry
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Microscopy, Electron
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Molecular Weight
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Periodicity
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Sensory Rhodopsins*
Substances
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Archaeal Proteins
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Halorhodopsins
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Lipid Bilayers
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Recombinant Fusion Proteins
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Sensory Rhodopsins
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sensory rhodopsin II protein, archaeal
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Carotenoids
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Histidine
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Bacteriorhodopsins