Helicobacter pylori and Epstein-Barr virus infection and the p53 tumour suppressor pathway in gastric stump cancer compared with carcinoma in the non-operated stomach

J Clin Pathol. 1998 Sep;51(9):662-6. doi: 10.1136/jcp.51.9.662.

Abstract

Aim: To evaluate similarities and differences between gastric stump cancer and conventional carcinoma in the non-operated stomach.

Methods: 26 stump carcinomas were compared with 24 conventional stomach cancers. Stage, histological type, and demographics were comparable in the two groups. Expression of p53 and p21-Waf1/Cip1 was evaluated by immunohistochemical staining. Helicobacter pylori infection was evaluated by examining haematoxylin-eosin stained slides and immunohistochemistry. Epstein-Barr virus infection was evaluated by RNA in situ hybridisation.

Results: Expression of p53 and p21-Waf1/Cip1 was similar in both groups and positive in more than half of the patients. H pylori infection was observed in six stump carcinomas and 17 conventional carcinomas in the intact stomach (p < 0.01). RNA in situ hybridisation (EBER1-ISH) for Epstein-Barr virus was positive in nine stump carcinomas and two carcinomas in the non-operated stomach (p < 0.05).

Conclusions: There appear to be aetiological differences between stump carcinoma and cancer in the intact stomach. Further study of these differences may improve our understanding of gastric carcinogenesis in general.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins / metabolism
  • Enzyme Inhibitors / metabolism
  • Epstein-Barr Virus Infections / complications*
  • Female
  • Gastric Stump
  • Helicobacter Infections / complications*
  • Helicobacter pylori*
  • Humans
  • In Situ Hybridization
  • Male
  • Middle Aged
  • Neoplasm Proteins / metabolism
  • RNA, Viral / analysis
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / virology*
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • Enzyme Inhibitors
  • Neoplasm Proteins
  • RNA, Viral
  • Tumor Suppressor Protein p53