pUCPCR1. A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3'-overhanging T residues

Mol Biotechnol. 1998 Dec;10(3):273-4. doi: 10.1007/BF02740849.

Abstract

The multiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion with XcmI gives a linear vector with a single 3'-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced by Taq polymerase can be directly cloned without further modifications.

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods*
  • DNA Restriction Enzymes / genetics
  • Genetic Vectors / genetics*
  • Molecular Sequence Data
  • Oligonucleotides / genetics
  • Polymerase Chain Reaction / methods*

Substances

  • Oligonucleotides
  • DNA Restriction Enzymes