Abstract
Galectin-1 and galectin-3 are galactoside-binding proteins involved in different steps of tumor progression and potential targets for therapy. We have investigated the expression of these galectins in 38 human bladder transitional-cell carcinomas of different histological grade and clinical stage and in 5 normal urothelium samples. Galectin-1 mRNA levels were highly increased in most high-grade tumors compared with normal bladder or low-grade tumors. Western blot and immuno-histochemical analysis of normal and neoplastic tissues revealed a higher content of galectin-1 in tumors. Galectin-3 mRNA levels were also increased in most tumors compared with normal urothelium, but levels were comparable among tumors of different histological grade.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Antigens, Differentiation / biosynthesis*
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Antigens, Differentiation / genetics
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Blotting, Northern
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Blotting, Western
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Carcinoma, Transitional Cell / genetics
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Carcinoma, Transitional Cell / metabolism*
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Carcinoma, Transitional Cell / pathology
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Female
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Galectin 1
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Galectin 3
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Hemagglutinins / biosynthesis*
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Hemagglutinins / genetics
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Humans
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Immunohistochemistry
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Male
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Middle Aged
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RNA, Messenger / analysis
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Urethra / metabolism
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Urinary Bladder / metabolism
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Urinary Bladder Neoplasms / genetics
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Urinary Bladder Neoplasms / metabolism*
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Urinary Bladder Neoplasms / pathology
Substances
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Antigens, Differentiation
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Galectin 1
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Galectin 3
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Hemagglutinins
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RNA, Messenger