The relationship of timing of early parental separation to psychosocial development of daughters was examined in 77 girls, 6-9 years of age over a four-year period. Disruptive behavior across contexts was prevalent in girls separated between birth and two years of age. Girls separated between three and five years of age showed more externalizing behavior problems, but only in school. Results suggest that early parental separation has more pervasive and stable negative effects on psychosocial adjustment of girls in this age group in single-parent families. Implications of the findings and directions for research are discussed.