Synthesis and evaluation of anthranilic acid-based transthyretin amyloid fibril inhibitors

Bioorg Med Chem Lett. 1999 Jan 4;9(1):1-6. doi: 10.1016/s0960-894x(98)00696-9.

Abstract

Eight small molecules were synthesized to evaluate the structure activity relationships (SAR) of N-substituted anthranilic acids. The molecules were synthesized by benzylation or arylation of methyl anthranilate. A light scattering-based amyloid fibril formation assay was used to evaluate potential inhibitors of transthyretin (TTR) amyloid fibril formation in vitro. The m-carboxyphenylated and o-trifluoromethylphenylated anthranilic acids are potent inhibitors that will be subjected to further SAR and structural analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amyloid / antagonists & inhibitors*
  • Binding Sites
  • Drug Evaluation, Preclinical
  • Flufenamic Acid / chemistry*
  • Flufenamic Acid / metabolism
  • Flufenamic Acid / pharmacology*
  • Humans
  • Light
  • Molecular Structure
  • Prealbumin / drug effects*
  • Prealbumin / metabolism*
  • Protein Conformation
  • Protein Folding
  • Scattering, Radiation
  • Structure-Activity Relationship
  • ortho-Aminobenzoates / chemistry*
  • ortho-Aminobenzoates / metabolism
  • ortho-Aminobenzoates / pharmacology*

Substances

  • Amyloid
  • Prealbumin
  • ortho-Aminobenzoates
  • N-(3-carboxyphenyl)anthranilic acid
  • Flufenamic Acid