Subcellular fractionation of polarized epithelial cells and identification of organelle-specific proteins by two-dimensional gel electrophoresis.
Fialka I, Pasquali C, Lottspeich F, Ahorn H, Huber LA.
Fialka I, et al. Among authors: pasquali c.
Electrophoresis. 1997 Dec;18(14):2582-90. doi: 10.1002/elps.1150181414.
Electrophoresis. 1997.
PMID: 9527488
Based on improved sample preparation and loading techniques (this issue, C. Pasquali et al., Electrophoresis, 1997, 18, 2573-2581), we were able to identify several proteins by immunoblotting or microsequencing of Coomassie-stained spots. ...
Based on improved sample preparation and loading techniques (this issue, C. Pasquali et al., Electrophoresis, 1997, 18, 2573-2 …