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A novel mass spectrometric assay for the cerebroside sulfate activator protein (saposin B) and arylsulfatase A.
Norris AJ, Whitelegge JP, Yaghoubian A, Alattia JR, Privé GG, Toyokuni T, Sun H, Brooks MN, Panza L, Matto P, Compostella F, Remmel N, Klingenstein R, Sandhoff K, Fluharty C, Fluharty A, Faull KF. Norris AJ, et al. J Lipid Res. 2005 Oct;46(10):2254-64. doi: 10.1194/jlr.M500188-JLR200. Epub 2005 Aug 1. J Lipid Res. 2005. PMID: 16061947 Free article.
Disulfide connectivity in cerebroside sulfate activator is not necessary for biological activity or alpha-helical content but is necessary for trypsin resistance and strong ligand binding.
Faull KF, Higginson J, Waring AJ, Johnson J, To T, Whitelegge JP, Stevens RL, Fluharty CB, Fluharty AL. Faull KF, et al. Among authors: fluharty al. Arch Biochem Biophys. 2000 Apr 15;376(2):266-74. doi: 10.1006/abbi.2000.1714. Arch Biochem Biophys. 2000. PMID: 10775412
106 results