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Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6.
Raffini LJ, Slater DJ, Rappaport EF, Lo Nigro L, Cheung NK, Biegel JA, Nowell PC, Lange BJ, Felix CA. Raffini LJ, et al. Among authors: rappaport ef. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4568-73. doi: 10.1073/pnas.062066799. Proc Natl Acad Sci U S A. 2002. PMID: 11930009 Free PMC article.
Reciprocal DNA topoisomerase II cleavage events at 5'-TATTA-3' sequences in MLL and AF-9 create homologous single-stranded overhangs that anneal to form der(11) and der(9) genomic breakpoint junctions in treatment-related AML without further processing.
Whitmarsh RJ, Saginario C, Zhuo Y, Hilgenfeld E, Rappaport EF, Megonigal MD, Carroll M, Liu M, Osheroff N, Cheung NK, Slater DJ, Ried T, Knutsen T, Blair IA, Felix CA. Whitmarsh RJ, et al. Among authors: rappaport ef. Oncogene. 2003 Nov 20;22(52):8448-59. doi: 10.1038/sj.onc.1207052. Oncogene. 2003. PMID: 14627986
t(11;22)(q23;q11.2) In acute myeloid leukemia of infant twins fuses MLL with hCDCrel, a cell division cycle gene in the genomic region of deletion in DiGeorge and velocardiofacial syndromes.
Megonigal MD, Rappaport EF, Jones DH, Williams TM, Lovett BD, Kelly KM, Lerou PH, Moulton T, Budarf ML, Felix CA. Megonigal MD, et al. Among authors: rappaport ef. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6413-8. doi: 10.1073/pnas.95.11.6413. Proc Natl Acad Sci U S A. 1998. PMID: 9600980 Free PMC article.
Duplicated regions of AF-4 intron 4 at t(4;11) translocation breakpoints.
Felix CA, Hosler MR, Slater DJ, Megonigal MD, Lovett BD, Williams TM, Nowell PC, Spinner NB, Owens NL, Hoxie J, Croce CM, Lange BJ, Rappaport EF. Felix CA, et al. Among authors: rappaport ef. Mol Diagn. 1999 Dec;4(4):269-83. doi: 10.1016/s1084-8592(99)80002-2. Mol Diagn. 1999. PMID: 10671636
129 results