In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes.
Fuchs BM, Syutsubo K, Ludwig W, Amann R.
Fuchs BM, et al.
Appl Environ Microbiol. 2001 Feb;67(2):961-8. doi: 10.1128/AEM.67.2.961-968.2001.
Appl Environ Microbiol. 2001.
PMID: 11157269
Free PMC article.
One of the main causes of failure of fluorescence in situ hybridization with rRNA-targeted oligonucleotides, besides low cellular ribosome content and impermeability of cell walls, is the inaccessibility of probe target sites due to higher-order structure of the ribosome. Analogo …
One of the main causes of failure of fluorescence in situ hybridization with rRNA-targeted oligonucleotides, besides low cellular ribosome c …