Site-directed mutagenesis of a possible type 1 copper ligand of bilirubin oxidase; a Met467Gln mutant shows stellacyanin-like properties.
Shimizu A, Sasaki T, Kwon JH, Odaka A, Satoh T, Sakurai N, Sakurai T, Yamaguchi S, Samejima T.
Shimizu A, et al.
J Biochem. 1999 Apr;125(4):662-8. doi: 10.1093/oxfordjournals.jbchem.a022334.
J Biochem. 1999.
PMID: 10101277
Free article.
In our previous paper, we reported a mutant of recombinant Myrothecium verrucaria bilirubin oxidase, in which the Met467 residue was replaced by Gly [Shimizu, A. et al. (1999) Biochemistry 38, 3034-3042]. ...Met467His and Met467Arg show no enzymatic activity …
In our previous paper, we reported a mutant of recombinant Myrothecium verrucaria bilirubin oxidase, in which the Met467 residue was …