Humphreys DP - Search Results

1

A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis.

Humphreys DP, Carrington B, Bowering LC, Ganesh R, Sehdev M, Smith BJ, King LM, Reeks DG, Lawson A, Popplewell AG. Humphreys DP, et al. Protein Expr Purif. 2002 Nov;26(2):309-20. doi: 10.1016/s1046-5928(02)00543-0. Protein Expr Purif. 2002. PMID: 12406686

2

Co-expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed in Escherichia coli.

Humphreys DP, Weir N, Lawson A, Mountain A, Lund PA. Humphreys DP, et al. FEBS Lett. 1996 Feb 12;380(1-2):194-7. doi: 10.1016/0014-5793(96)00028-2. FEBS Lett. 1996. PMID: 8603736 Free article.

3

Formation of dimeric Fabs in Escherichia coli: effect of hinge size and isotype, presence of interchain disulphide bond, Fab' expression levels, tail piece sequences and growth conditions.

Humphreys DP, Chapman AP, Reeks DG, Lang V, Stephens PE. Humphreys DP, et al. J Immunol Methods. 1997 Dec 1;209(2):193-202. doi: 10.1016/s0022-1759(97)00172-5. J Immunol Methods. 1997. PMID: 9461335

4

F(ab')2 molecules made from Escherichia coli produced Fab' with hinge sequences conferring increased serum survival in an animal model.

Humphreys DP, Vetterlein OM, Chapman AP, King DJ, Antoniw P, Suitters AJ, Reeks DG, Parton TA, King LM, Smith BJ, Lang V, Stephens PE. Humphreys DP, et al. J Immunol Methods. 1998 Aug 1;217(1-2):1-10. doi: 10.1016/s0022-1759(98)00061-1. J Immunol Methods. 1998. PMID: 9776570

5

Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage.

Humphreys DP, Smith BJ, King LM, West SM, Reeks DG, Stephens PE. Humphreys DP, et al. Protein Eng. 1999 Feb;12(2):179-84. doi: 10.1093/protein/12.2.179. Protein Eng. 1999. PMID: 10195290

6

Improved efficiency of site-specific copper(II) ion-catalysed protein cleavage effected by mutagenesis of cleavage site.

Humphreys DP, King LM, West SM, Chapman AP, Sehdev M, Redden MW, Glover DJ, Smith BJ, Stephens PE. Humphreys DP, et al. Protein Eng. 2000 Mar;13(3):201-6. doi: 10.1093/protein/13.3.201. Protein Eng. 2000. PMID: 10775662

7

High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence.

Humphreys DP, Sehdev M, Chapman AP, Ganesh R, Smith BJ, King LM, Glover DJ, Reeks DG, Stephens PE. Humphreys DP, et al. Protein Expr Purif. 2000 Nov;20(2):252-64. doi: 10.1006/prep.2000.1286. Protein Expr Purif. 2000. PMID: 11049749

8

Production of antibodies and antibody fragments in Escherichia coli and a comparison of their functions, uses and modification.

Humphreys DP. Humphreys DP. Curr Opin Drug Discov Devel. 2003 Mar;6(2):188-96. Curr Opin Drug Discov Devel. 2003. PMID: 12669454 Review.

9

Engineering of Escherichia coli to improve the purification of periplasmic Fab' fragments: changing the pI of the chromosomally encoded PhoS/PstS protein.

Humphreys DP, Heywood SP, King LM, Bowering LC, Turner JP, Lane SE. Humphreys DP, et al. Protein Expr Purif. 2004 Sep;37(1):109-18. doi: 10.1016/j.pep.2004.04.027. Protein Expr Purif. 2004. PMID: 15294288

10

Alternative antibody Fab' fragment PEGylation strategies: combination of strong reducing agents, disruption of the interchain disulphide bond and disulphide engineering.

Humphreys DP, Heywood SP, Henry A, Ait-Lhadj L, Antoniw P, Palframan R, Greenslade KJ, Carrington B, Reeks DG, Bowering LC, West S, Brand HA. Humphreys DP, et al. Protein Eng Des Sel. 2007 May;20(5):227-34. doi: 10.1093/protein/gzm015. Epub 2007 Apr 23. Protein Eng Des Sel. 2007. PMID: 17452434

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