Kawarasaki Y - Search Results

1

Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.

Ikeuchi A, Kawarasaki Y, Shinbata T, Yamane T. Ikeuchi A, et al. Among authors: kawarasaki y. Biotechnol Prog. 2003 Sep-Oct;19(5):1460-7. doi: 10.1021/bp034029t. Biotechnol Prog. 2003. PMID: 14524707

2

A method for functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCR.

Kawarasaki Y, Sasaki Y, Ikeuchi A, Yamamoto S, Yamane T. Kawarasaki Y, et al. Anal Biochem. 2002 Apr 1;303(1):34-41. doi: 10.1006/abio.2001.5569. Anal Biochem. 2002. PMID: 11906148

3

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.

Rungpragayphan S, Kawarasaki Y, Imaeda T, Kohda K, Nakano H, Yamane T. Rungpragayphan S, et al. Among authors: kawarasaki y. J Mol Biol. 2002 Apr 26;318(2):395-405. doi: 10.1016/S0022-2836(02)00094-3. J Mol Biol. 2002. PMID: 12051846

4

Cell-free protein synthesis systems: increasing their performance and applications.

Nakano H, Kawarasaki Y, Yamane T. Nakano H, et al. Among authors: kawarasaki y. Adv Biochem Eng Biotechnol. 2004;90:135-49. doi: 10.1007/b94195. Adv Biochem Eng Biotechnol. 2004. PMID: 15453188 Review.

5

A trimmed viral cap-independent translation enhancing sequence for rapid in vitro gene expression.

Kawarasaki Y, Kasahara S, Kodera N, Shinbata T, Sekiguchi S, Nakano H, Yamane T. Kawarasaki Y, et al. Biotechnol Prog. 2000 May-Jun;16(3):517-21. doi: 10.1021/bp000021x. Biotechnol Prog. 2000. PMID: 10835258

6

Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.

Ikeuchi A, Sasaki Y, Kawarasaki Y, Yamane T. Ikeuchi A, et al. Among authors: kawarasaki y. Nucleic Acids Res. 2003 Dec 1;31(23):6953-62. doi: 10.1093/nar/gkg888. Nucleic Acids Res. 2003. PMID: 14627828 Free PMC article.

7

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets.

Kojima T, Takei Y, Ohtsuka M, Kawarasaki Y, Yamane T, Nakano H. Kojima T, et al. Among authors: kawarasaki y. Nucleic Acids Res. 2005 Oct 6;33(17):e150. doi: 10.1093/nar/gni143. Nucleic Acids Res. 2005. PMID: 16214800 Free PMC article.

8

A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression.

Ikeuchi A, Nakano H, Kamiya T, Yamane T, Kawarasaki Y. Ikeuchi A, et al. Among authors: kawarasaki y. Biotechnol Prog. 2010 Jul-Aug;26(4):945-53. doi: 10.1002/btpr.403. Biotechnol Prog. 2010. PMID: 20730753

9

Phosphatase-immunodepleted cell-free protein synthesis system.

Kawarasaki Y, Nakano H, Yamane T. Kawarasaki Y, et al. J Biotechnol. 1998 May 13;61(3):199-208. doi: 10.1016/s0168-1656(98)00033-9. J Biotechnol. 1998. PMID: 9684338

10

Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract.

Kawarasaki Y, Yamada Y, Ichimori M, Shinbata T, Kohda K, Nakano H, Yamane T. Kawarasaki Y, et al. J Biosci Bioeng. 2003;95(3):209-14. J Biosci Bioeng. 2003. PMID: 16233395

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