26 kDa endochitinase from barley seeds: an interaction of the ionizable side chains essential for catalysis.
Ohnishi T, Juffer AH, Tamoi M, Skriver K, Fukamizo T.
Ohnishi T, et al. Among authors: fukamizo t.
J Biochem. 2005 Nov;138(5):553-62. doi: 10.1093/jb/mvi154.
J Biochem. 2005.
PMID: 16272567
The transition temperature of thermal unfolding (T(m)) of R215A was lower than that of the wild type protein by about 6.2 degrees C. ...A similar interaction network was previously found in chitosanase from Streptomyces sp. N174 [Fukamizo et al. (2000) J. Biol. Chem …
The transition temperature of thermal unfolding (T(m)) of R215A was lower than that of the wild type protein by about 6.2 degrees C. …