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Sequence-specific Ni(II)-dependent peptide bond hydrolysis for protein engineering. Combinatorial library determination of optimal sequences.
Krezel A, Kopera E, Protas AM, Poznański J, Wysłouch-Cieszyńska A, Bal W. Krezel A, et al. Among authors: kopera e. J Am Chem Soc. 2010 Mar 17;132(10):3355-66. doi: 10.1021/ja907567r. J Am Chem Soc. 2010. PMID: 20166730
Previously we demonstrated for several examples that peptides having a general internal sequence R(N)-Yaa-Ser/Thr-Xaa-His-Zaa-R(C) (Yaa = Glu or Ala, Xaa = Ala or His, Zaa = Lys, R(N) and R(C) = any N- and C-terminal amino acid sequence) were hydrolyzed specifically at the Yaa-Se …
Previously we demonstrated for several examples that peptides having a general internal sequence R(N)-Yaa-Ser/Thr-Xaa-His-Zaa-R(C) (Yaa = Gl …
Sequence-specific Ni(II)-dependent peptide bond hydrolysis for protein engineering: reaction conditions and molecular mechanism.
Kopera E, Krezel A, Protas AM, Belczyk A, Bonna A, Wysłouch-Cieszyńska A, Poznański J, Bal W. Kopera E, et al. Inorg Chem. 2010 Jul 19;49(14):6636-45. doi: 10.1021/ic1005709. Inorg Chem. 2010. PMID: 20550138
Recently we screened a combinatorial library of R(1)-(Ser/Thr)-Xaa-His-Zaa-R(2) peptides (Xaa = 17 common alpha-amino acids, except Asp, Glu, and Cys; Zaa =19 common alpha-amino acids, except Cys; R(1) = CH(3)CO-Gly-Ala, R(2) = Lys-Phe-Leu-NH(2)) and established criteria for sele …
Recently we screened a combinatorial library of R(1)-(Ser/Thr)-Xaa-His-Zaa-R(2) peptides (Xaa = 17 common alpha-amino acids, except Asp, Glu …
Selective peptide bond hydrolysis of cysteine peptides in the presence of Ni(II) ions.
Protas AM, Bonna A, Kopera E, Bal W. Protas AM, et al. Among authors: kopera e. J Inorg Biochem. 2011 Jan;105(1):10-6. doi: 10.1016/j.jinorgbio.2010.09.003. Epub 2010 Sep 29. J Inorg Biochem. 2011. PMID: 21134597
Recently, we described a sequence-specific R1-(Ser/Thr) peptide bond hydrolysis reaction in peptides of a general sequence R1-(Ser/Thr)-Xaa-His-Zaa-R, which occurs in the presence of Ni(II) ions [A. Krezel, E. Kopera, A. M. Protas, A. Wyslouch-Cieszynska, J. Poznans …
Recently, we described a sequence-specific R1-(Ser/Thr) peptide bond hydrolysis reaction in peptides of a general sequence R1-(Ser/Thr)-Xaa- …
A zinc-finger like metal binding site in the nucleosome.
Adamczyk M, Poznański J, Kopera E, Bal W. Adamczyk M, et al. Among authors: kopera e. FEBS Lett. 2007 Apr 3;581(7):1409-16. doi: 10.1016/j.febslet.2007.02.063. Epub 2007 Mar 5. FEBS Lett. 2007. PMID: 17350622 Free article.
Reaction of the XPA zinc finger with S-nitrosoglutathione.
Smirnova J, Zhukova L, Witkiewicz-Kucharczyk A, Kopera E, Oledzki J, Wysłouch-Cieszyńska A, Palumaa P, Hartwig A, Bal W. Smirnova J, et al. Among authors: kopera e. Chem Res Toxicol. 2008 Feb;21(2):386-92. doi: 10.1021/tx700297f. Epub 2008 Jan 3. Chem Res Toxicol. 2008. PMID: 18171019
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