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Identification of the active site residues in dipeptidyl peptidase IV by affinity labeling and site-directed mutagenesis.
Ogata S, Misumi Y, Tsuji E, Takami N, Oda K, Ikehara Y. Ogata S, et al. Among authors: ikehara y. Biochemistry. 1992 Mar 10;31(9):2582-7. doi: 10.1021/bi00124a019. Biochemistry. 1992. PMID: 1347701
A comparison of the determined sequence with the predicted primary structure of DPPIV [Ogata, S., Misumi, Y., & Ikehara, Y. (1989) J. Biol. Chem. 264, 3596-3601] revealed that [3H]DFP was bound to Ser631 within the sequence Gly629-Trp-Ser-Tyr-Gly633, whic …
A comparison of the determined sequence with the predicted primary structure of DPPIV [Ogata, S., Misumi, Y., & Ikehara, …
An active-site mutation (Gly633-->Arg) of dipeptidyl peptidase IV causes its retention and rapid degradation in the endoplasmic reticulum.
Tsuji E, Misumi Y, Fujiwara T, Takami N, Ogata S, Ikehara Y. Tsuji E, et al. Among authors: ikehara y. Biochemistry. 1992 Dec 1;31(47):11921-7. doi: 10.1021/bi00162a035. Biochemistry. 1992. PMID: 1359907
Cloning and sequencing of DPPIV cDNAs revealed a G to A transition at nucleotide 1897 in the cDNA sequence of 344/CRJ, which leads to substitution of Gly633-->Arg in the active-site sequence Gly629-Trp-Ser-Tyr-Gly633 determined for the wild-type DPPIV [Ogata, S., Misumi, Y …
Cloning and sequencing of DPPIV cDNAs revealed a G to A transition at nucleotide 1897 in the cDNA sequence of 344/CRJ, which leads to substi …
Membrane-anchoring domain of rat liver 5'-nucleotidase: identification of the COOH-terminal serine-523 covalently attached with a glycolipid.
Ogata S, Hayashi Y, Misumi Y, Ikehara Y. Ogata S, et al. Among authors: ikehara y. Biochemistry. 1990 Aug 28;29(34):7923-7. doi: 10.1021/bi00486a021. Biochemistry. 1990. PMID: 2148114
The sequences of the tetradeca- and dipeptides thus determined are identified at positions 510-523 and 522-523, respectively, in the primary structure deduced from the cDNA sequence, which predicts a further extension to position 548, containing a hydrophobic amino acid sequence …
The sequences of the tetradeca- and dipeptides thus determined are identified at positions 510-523 and 522-523, respectively, in the primary …
345 results