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Engineering a camelid antibody fragment that binds to the active site of human lysozyme and inhibits its conversion into amyloid fibrils.
Chan PH, Pardon E, Menzer L, De Genst E, Kumita JR, Christodoulou J, Saerens D, Brans A, Bouillenne F, Archer DB, Robinson CV, Muyldermans S, Matagne A, Redfield C, Wyns L, Dobson CM, Dumoulin M. Chan PH, et al. Among authors: dumoulin m. Biochemistry. 2008 Oct 21;47(42):11041-54. doi: 10.1021/bi8005797. Epub 2008 Sep 25. Biochemistry. 2008. PMID: 18816062
A non-natural variant of human lysozyme (I59T) mimics the in vitro behaviour of the I56T variant that is responsible for a form of familial amyloidosis.
Hagan CL, Johnson RJ, Dhulesia A, Dumoulin M, Dumont J, De Genst E, Christodoulou J, Robinson CV, Dobson CM, Kumita JR. Hagan CL, et al. Among authors: dumoulin m. Protein Eng Des Sel. 2010 Jul;23(7):499-506. doi: 10.1093/protein/gzq023. Epub 2010 Apr 9. Protein Eng Des Sel. 2010. PMID: 20382744 Free PMC article.
Structure and properties of a complex of α-synuclein and a single-domain camelid antibody.
De Genst EJ, Guilliams T, Wellens J, O'Day EM, Waudby CA, Meehan S, Dumoulin M, Hsu ST, Cremades N, Verschueren KH, Pardon E, Wyns L, Steyaert J, Christodoulou J, Dobson CM. De Genst EJ, et al. Among authors: dumoulin m. J Mol Biol. 2010 Sep 17;402(2):326-43. doi: 10.1016/j.jmb.2010.07.001. Epub 2010 Jul 8. J Mol Biol. 2010. PMID: 20620148 Free article.
162 results