Wagner R - Search Results

1

A novel, generic and effective method for the rapid purification of G protein-coupled receptors.

Magnin T, Fiez-Vandal C, Potier N, Coquard A, Leray I, Steffan T, Logez C, Alkhalfioui F, Pattus F, Wagner R. Magnin T, et al. Among authors: wagner r. Protein Expr Purif. 2009 Mar;64(1):1-7. doi: 10.1016/j.pep.2008.09.007. Epub 2008 Sep 19. Protein Expr Purif. 2009. PMID: 18835448

2

Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells: a potential expression system for large-scale production of G-protein coupled receptors.

Perret BG, Wagner R, Lecat S, Brillet K, Rabut G, Bucher B, Pattus F. Perret BG, et al. Among authors: wagner r. Protein Expr Purif. 2003 Sep;31(1):123-32. doi: 10.1016/s1046-5928(03)00140-2. Protein Expr Purif. 2003. PMID: 12963349

3

Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells.

Hassaine G, Wagner R, Kempf J, Cherouati N, Hassaine N, Prual C, André N, Reinhart C, Pattus F, Lundstrom K. Hassaine G, et al. Among authors: wagner r. Protein Expr Purif. 2006 Feb;45(2):343-51. doi: 10.1016/j.pep.2005.06.007. Epub 2005 Jul 11. Protein Expr Purif. 2006. PMID: 16055346

4

Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen.

André N, Cherouati N, Prual C, Steffan T, Zeder-Lutz G, Magnin T, Pattus F, Michel H, Wagner R, Reinhart C. André N, et al. Among authors: wagner r. Protein Sci. 2006 May;15(5):1115-26. doi: 10.1110/ps.062098206. Epub 2006 Apr 5. Protein Sci. 2006. PMID: 16597836 Free PMC article.

5

Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris.

Zeder-Lutz G, Cherouati N, Reinhart C, Pattus F, Wagner R. Zeder-Lutz G, et al. Among authors: wagner r. Protein Expr Purif. 2006 Nov;50(1):118-27. doi: 10.1016/j.pep.2006.05.017. Epub 2006 Jun 8. Protein Expr Purif. 2006. PMID: 16843005

6

Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.

Lundstrom K, Wagner R, Reinhart C, Desmyter A, Cherouati N, Magnin T, Zeder-Lutz G, Courtot M, Prual C, André N, Hassaine G, Michel H, Cambillau C, Pattus F. Lundstrom K, et al. Among authors: wagner r. J Struct Funct Genomics. 2006 Jun;7(2):77-91. doi: 10.1007/s10969-006-9011-2. Epub 2006 Nov 22. J Struct Funct Genomics. 2006. PMID: 17120110

7

Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures.

Singh S, Gras A, Fiez-Vandal C, Ruprecht J, Rana R, Martinez M, Strange PG, Wagner R, Byrne B. Singh S, et al. Among authors: wagner r. Microb Cell Fact. 2008 Oct 10;7:28. doi: 10.1186/1475-2859-7-28. Microb Cell Fact. 2008. PMID: 18847468 Free PMC article.

8

Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells.

Brillet K, Perret BG, Klein V, Pattus F, Wagner R. Brillet K, et al. Among authors: wagner r. Cytotechnology. 2008 May;57(1):101-9. doi: 10.1007/s10616-008-9125-4. Epub 2008 Jan 19. Cytotechnology. 2008. PMID: 19003178 Free PMC article.

9

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies.

Michalke K, Gravière ME, Huyghe C, Vincentelli R, Wagner R, Pattus F, Schroeder K, Oschmann J, Rudolph R, Cambillau C, Desmyter A. Michalke K, et al. Among authors: wagner r. Anal Biochem. 2009 Mar 15;386(2):147-55. doi: 10.1016/j.ab.2008.12.016. Epub 2008 Dec 24. Anal Biochem. 2009. PMID: 19150325

10

From purified GPCRs to drug discovery: the promise of protein-based methodologies.

Alkhalfioui F, Magnin T, Wagner R. Alkhalfioui F, et al. Among authors: wagner r. Curr Opin Pharmacol. 2009 Oct;9(5):629-35. doi: 10.1016/j.coph.2009.04.002. Epub 2009 May 13. Curr Opin Pharmacol. 2009. PMID: 19443270 Review.

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