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Heterologous overexpression and purification of cytochrome c' from Rhodobacter capsulatus and a mutant (K42E) in the dimerization region. Mutation does not alter oligomerization but impacts the heme iron spin state and nitric oxide binding properties.
Huston WM, Andrew CR, Servid AE, McKay AL, Leech AP, Butler CS, Moir JW. Huston WM, et al. Among authors: servid ae. Biochemistry. 2006 Apr 11;45(14):4388-95. doi: 10.1021/bi052605j. Biochemistry. 2006. PMID: 16584174