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Effect of propan-2-ol on enzymic and structural properties of elongation factor G.
Masullo M, Parlato G, De Vendittis E, Bocchini V. Masullo M, et al. Biochem J. 1989 Aug 1;261(3):725-31. doi: 10.1042/bj2610725. Biochem J. 1989. PMID: 2552989 Free PMC article.
Elongation factor G (EF-G) can support a GTPase activity in vitro even in the absence of ribosomes when propan-2-ol is present [GTPasep; De Vendittis, Masullo & Bocchini (1986) J. Biol. Chem. 261, 4445-4450]. In the present work the GTPasep activity of EF-G was further …
Elongation factor G (EF-G) can support a GTPase activity in vitro even in the absence of ribosomes when propan-2-ol is present [GTPasep; De …
The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus.
De Vendittis E, Adinolfi BS, Amatruda MR, Raimo G, Masullo M, Bocchini V. De Vendittis E, et al. Among authors: masullo m. Eur J Biochem. 1999 Jun;262(2):600-5. doi: 10.1046/j.1432-1327.1999.00428.x. Eur J Biochem. 1999. PMID: 10336648 Free article.
In contrast, the A26G substitution enhanced the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol [Raimo, G., Masullo, M., Scarano, G., & Bocchini, V. (1997) Biochimie 78, 832-837]. Surprisingly, A26GSsEF-2 was able to hydrolyse GT …
In contrast, the A26G substitution enhanced the catalytic efficiency of the intrinsic SsEF-2 GTPase triggered by ethylene glycol [Raimo, G., …
Phenylmethanesulfonyl fluoride inactivates an archaeal superoxide dismutase by chemical modification of a specific tyrosine residue. Cloning, sequencing and expression of the gene coding for Sulfolobus solfataricus superoxide dismutase.
De Vendittis E, Ursby T, Rullo R, Gogliettino MA, Masullo M, Bocchini V. De Vendittis E, et al. Among authors: masullo m. Eur J Biochem. 2001 Mar;268(6):1794-801. doi: 10.1046/j.1432-1327.2001.02052.x. Eur J Biochem. 2001. PMID: 11248699 Free article.
260 results