Nakade S - Search Results

1

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9.

Nakade S, Tsubota T, Sakane Y, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T, Suzuki KT. Nakade S, et al. Nat Commun. 2014 Nov 20;5:5560. doi: 10.1038/ncomms6560. Nat Commun. 2014. PMID: 25410609 Free PMC article.

2

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish.

Hisano Y, Sakuma T, Nakade S, Ohga R, Ota S, Okamoto H, Yamamoto T, Kawahara A. Hisano Y, et al. Among authors: nakade s. Sci Rep. 2015 Mar 5;5:8841. doi: 10.1038/srep08841. Sci Rep. 2015. PMID: 25740433 Free PMC article.

3

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T. Sakuma T, et al. Among authors: nakade s. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Nat Protoc. 2016. PMID: 26678082

4

Cas9, Cpf1 and C2c1/2/3-What's next?

Nakade S, Yamamoto T, Sakuma T. Nakade S, et al. Bioengineered. 2017 May 4;8(3):265-273. doi: 10.1080/21655979.2017.1282018. Epub 2017 Jan 31. Bioengineered. 2017. PMID: 28140746 Free PMC article. Review.

5

Biased genome editing using the local accumulation of DSB repair molecules system.

Nakade S, Mochida K, Kunii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T, Yamamoto T. Nakade S, et al. Nat Commun. 2018 Aug 16;9(1):3270. doi: 10.1038/s41467-018-05773-6. Nat Commun. 2018. PMID: 30115916 Free PMC article.

6

Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs.

Nakade S, Sakuma T, Sakane Y, Hara Y, Kurabayashi A, Kashiwagi K, Kashiwagi A, Yamamoto T, Obara M. Nakade S, et al. In Vitro Cell Dev Biol Anim. 2015 Oct;51(9):879-84. doi: 10.1007/s11626-015-9912-0. Epub 2015 Apr 29. In Vitro Cell Dev Biol Anim. 2015. PMID: 25920501

7

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ.

Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K. Aida T, et al. Among authors: nakade s. BMC Genomics. 2016 Nov 28;17(1):979. doi: 10.1186/s12864-016-3331-9. BMC Genomics. 2016. PMID: 27894274 Free PMC article.

8

Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO.

Nakamae K, Nishimura Y, Takenaga M, Nakade S, Sakamoto N, Ide H, Sakuma T, Yamamoto T. Nakamae K, et al. Among authors: nakade s. Bioengineered. 2017 May 4;8(3):302-308. doi: 10.1080/21655979.2017.1313645. Epub 2017 Apr 28. Bioengineered. 2017. PMID: 28453368 Free PMC article.

9

Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus.

Sakuma T, Mochida K, Nakade S, Ezure T, Minagawa S, Yamamoto T. Sakuma T, et al. Among authors: nakade s. Genes Cells. 2018 Apr;23(4):255-263. doi: 10.1111/gtc.12569. Epub 2018 Feb 9. Genes Cells. 2018. PMID: 29423928 Free article.

10

Cancer induction and suppression with transcriptional control and epigenome editing technologies.

Nakade S, Yamamoto T, Sakuma T. Nakade S, et al. J Hum Genet. 2018 Feb;63(2):187-194. doi: 10.1038/s10038-017-0377-8. Epub 2017 Nov 14. J Hum Genet. 2018. PMID: 29215091 Review.

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