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Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.
Michalke K, Huyghe C, Lichière J, Gravière ME, Siponen M, Sciara G, Lepaul I, Wagner R, Magg C, Rudolph R, Cambillau C, Desmyter A. Michalke K, et al. Among authors: wagner r. Anal Biochem. 2010 Jun 1;401(1):74-80. doi: 10.1016/j.ab.2010.02.017. Epub 2010 Feb 20. Anal Biochem. 2010. PMID: 20175983
Overexpression of membrane proteins using Pichia pastoris.
Bornert O, Alkhalfioui F, Logez C, Wagner R. Bornert O, et al. Among authors: wagner r. Curr Protoc Protein Sci. 2012 Feb;Chapter 29:29.2.1-29.2.24. doi: 10.1002/0471140864.ps2902s67. Curr Protoc Protein Sci. 2012. PMID: 22294327
Preparation of Pichia pastoris expression plasmids.
Logez C, Alkhalfioui F, Byrne B, Wagner R. Logez C, et al. Among authors: wagner r. Methods Mol Biol. 2012;866:25-40. doi: 10.1007/978-1-61779-770-5_3. Methods Mol Biol. 2012. PMID: 22454111
GHSR-D2R heteromerization modulates dopamine signaling through an effect on G protein conformation.
Damian M, Pons V, Renault P, M'Kadmi C, Delort B, Hartmann L, Kaya AI, Louet M, Gagne D, Ben Haj Salah K, Denoyelle S, Ferry G, Boutin JA, Wagner R, Fehrentz JA, Martinez J, Marie J, Floquet N, Galès C, Mary S, Hamm HE, Banères JL. Damian M, et al. Among authors: wagner r. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4501-4506. doi: 10.1073/pnas.1712725115. Epub 2018 Apr 9. Proc Natl Acad Sci U S A. 2018. PMID: 29632174 Free PMC article.
VHH characterization. Comparison of recombinant with chemically synthesized anti-HER2 VHH.
Hartmann L, Botzanowski T, Galibert M, Jullian M, Chabrol E, Zeder-Lutz G, Kugler V, Stojko J, Strub JM, Ferry G, Frankiewicz L, Puget K, Wagner R, Cianférani S, Boutin JA. Hartmann L, et al. Among authors: wagner r. Protein Sci. 2019 Oct;28(10):1865-1879. doi: 10.1002/pro.3712. Epub 2019 Aug 29. Protein Sci. 2019. PMID: 31423659 Free PMC article.
4,104 results