Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase.
Xue B, Chow JY, Baldansuren A, Yap LL, Gan YH, Dikanov SA, Robinson RC, Yew WS.
Xue B, et al.
Biochemistry. 2013 Apr 2;52(13):2359-70. doi: 10.1021/bi4000904. Epub 2013 Mar 19.
Biochemistry. 2013.
PMID: 23461395
Free PMC article.
The in vitro evolution and engineering of quorum-quenching lactonases with enhanced reactivities was achieved using a thermostable GKL enzyme as a template, yielding the E101G/R230C GKL mutant with increased catalytic activity and a broadened substrate range [Chow, J. Y., Xue …
The in vitro evolution and engineering of quorum-quenching lactonases with enhanced reactivities was achieved using a thermostable GKL enzym …