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Novel purification of cytochrome c1 from mitochondrial Complex III. Reconstitution of antimycin-insensitive electron transfer with the iron-sulfur protein and cytochrome c1.
Shimomura Y, Nishikimi M, Ozawa T. Shimomura Y, et al. J Biol Chem. 1985 Dec 5;260(28):15075-80. J Biol Chem. 1985. PMID: 2999105 Free article.
Complex III of beef heart mitochondria was separated into the iron-sulfur protein and the complex devoid of it as described previously (Shimomura, Y., Nishikimi, M., and Ozawa, T. (1984) J. Biol. Chem. 259, 14059-14063). From the latter preparation, cytochrome c1 wa …
Complex III of beef heart mitochondria was separated into the iron-sulfur protein and the complex devoid of it as described previously (S …
Purification of the iron-sulfur protein, ubiquinone-binding protein, and cytochrome c1 from a single source of mitochondrial complex III.
Shimomura Y, Nishikimi M, Ozawa T. Shimomura Y, et al. Anal Biochem. 1986 Feb 15;153(1):126-31. doi: 10.1016/0003-2697(86)90070-9. Anal Biochem. 1986. PMID: 3008590
An iron-sulfur protein fraction was first eluted from the column with buffer containing 1% deoxycholate and no salt after removal of phospholipids from the complex by washing with the buffer for the column equilibration, as reported previously (Y. Shimomura, M. Nish …
An iron-sulfur protein fraction was first eluted from the column with buffer containing 1% deoxycholate and no salt after removal of phospho …
Integral polypeptide composition of Complex III of the mitochondrial electron-transfer chain.
Shimomura Y, Ozawa T. Shimomura Y, et al. Biochem Int. 1984 Jan;8(1):187-91. Biochem Int. 1984. PMID: 6089816
Further depletion of phospholipids to 0.6% makes the iron-sulfur protein dissociable from the complex, resulting in a loss of the enzymic activity (Shimomura, Y. and Ozawa, T. (1982) Biochem. Int. 5, 1-6). These results suggest that Complex III consists of 9 polypep …
Further depletion of phospholipids to 0.6% makes the iron-sulfur protein dissociable from the complex, resulting in a loss of the enzymic ac …
1,144 results