Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.
Garde A, Sherwood DR.
Garde A, et al.
STAR Protoc. 2022 May 26;3(2):101429. doi: 10.1016/j.xpro.2022.101429. eCollection 2022 Jun 17.
STAR Protoc. 2022.
PMID: 35664256
Free PMC article.
In this protocol, we describe how to quantify ATP levels using PercevalHR in C. elegans larvae during anchor cell invasion. PercevalHR is a fluorescent biosensor that reports the cytoplasmic ATP:ADP ratio. The protocol can be adapted to analyze the ATP:ADP ratios within ot …
In this protocol, we describe how to quantify ATP levels using PercevalHR in C. elegans larvae during anchor cell invasion. PercevalHR is …