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Multiple mutations in the aglycone binding pocket to convert the substrate specificity of dalcochinase to linamarase.
Biochem Biophys Res Commun. 2018 Oct 12;504(4):647-653. doi: 10.1016/j.bbrc.2018.08.202. Epub 2018 Sep 8.
Biochem Biophys Res Commun. 2018.
PMID: 30205957
Mutational analysis in the glycone binding pocket of Dalbergia cochinchinensis β-glucosidase to increase catalytic efficiency toward mannosides.
Ratananikom K, Choengpanya K, Tongtubtim N, Charoenrat T, Withers SG, Kongsaeree PT.
Ratananikom K, et al. Among authors: tongtubtim n.
Carbohydr Res. 2013 May 24;373:35-41. doi: 10.1016/j.carres.2012.10.018. Epub 2012 Oct 27.
Carbohydr Res. 2013.
PMID: 23583451
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Expression and purification of dalcochinase, a beta-glucosidase from Dalbergia cochinchinensis Pierre, in yeast and bacterial hosts.
Toonkool P, Metheenukul P, Sujiwattanarat P, Paiboon P, Tongtubtim N, Ketudat-Cairns M, Ketudat-Cairns J, Svasti J.
Toonkool P, et al. Among authors: tongtubtim n.
Protein Expr Purif. 2006 Aug;48(2):195-204. doi: 10.1016/j.pep.2006.05.011. Epub 2006 May 27.
Protein Expr Purif. 2006.
PMID: 16814564
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