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Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.
Ludwig AK, De Miroschedji K, Doeppner TR, Börger V, Ruesing J, Rebmann V, Durst S, Jansen S, Bremer M, Behrmann E, Singer BB, Jastrow H, Kuhlmann JD, El Magraoui F, Meyer HE, Hermann DM, Opalka B, Raunser S, Epple M, Horn PA, Giebel B. Ludwig AK, et al. Among authors: durst s. J Extracell Vesicles. 2018 Oct 17;7(1):1528109. doi: 10.1080/20013078.2018.1528109. eCollection 2018. J Extracell Vesicles. 2018. PMID: 30357008 Free PMC article.
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