Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils.
Romanowski G, Lorenz MG, Wackernagel W.
Romanowski G, et al. Among authors: lorenz mg.
Appl Environ Microbiol. 1993 Oct;59(10):3438-46. doi: 10.1128/aem.59.10.3438-3446.1993.
Appl Environ Microbiol. 1993.
PMID: 8250566
Free PMC article.
It was found that transformation by electroporation was about 20 times more efficient and much less inhibited by constituents of soil extracts than transformation of Ca(2+)-treated cells (G. Romanowski, M.G. Lorenz, G. Sayler, and W. Wackernagel, Appl. Environ. Micr …
It was found that transformation by electroporation was about 20 times more efficient and much less inhibited by constituents of soil extrac …