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Probabilistic cell-type assignment of single-cell RNA-seq for tumor microenvironment profiling.
Zhang AW, O'Flanagan C, Chavez EA, Lim JLP, Ceglia N, McPherson A, Wiens M, Walters P, Chan T, Hewitson B, Lai D, Mottok A, Sarkozy C, Chong L, Aoki T, Wang X, Weng AP, McAlpine JN, Aparicio S, Steidl C, Campbell KR, Shah SP. Zhang AW, et al. Among authors: hewitson b. Nat Methods. 2019 Oct;16(10):1007-1015. doi: 10.1038/s41592-019-0529-1. Epub 2019 Sep 9. Nat Methods. 2019. PMID: 31501550 Free PMC article.
Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses.
O'Flanagan CH, Campbell KR, Zhang AW, Kabeer F, Lim JLP, Biele J, Eirew P, Lai D, McPherson A, Kong E, Bates C, Borkowski K, Wiens M, Hewitson B, Hopkins J, Pham J, Ceglia N, Moore R, Mungall AJ, McAlpine JN; CRUK IMAXT Grand Challenge Team; Shah SP, Aparicio S. O'Flanagan CH, et al. Among authors: hewitson b. Genome Biol. 2019 Oct 17;20(1):210. doi: 10.1186/s13059-019-1830-0. Genome Biol. 2019. PMID: 31623682 Free PMC article.