Hollfelder F - Search Results

1

Ultrahigh-throughput screening in microfluidic droplets: a faster route to new enzymes.

Gantz M, Aleku GA, Hollfelder F. Gantz M, et al. Among authors: hollfelder f. Trends Biochem Sci. 2022 May;47(5):451-452. doi: 10.1016/j.tibs.2021.11.001. Epub 2021 Nov 27. Trends Biochem Sci. 2022. PMID: 34848125 No abstract available.

2

Microfluidic droplets: new integrated workflows for biological experiments.

Kintses B, van Vliet LD, Devenish SR, Hollfelder F. Kintses B, et al. Among authors: hollfelder f. Curr Opin Chem Biol. 2010 Oct;14(5):548-55. doi: 10.1016/j.cbpa.2010.08.013. Epub 2010 Sep 24. Curr Opin Chem Biol. 2010. PMID: 20869904 Review.

3

Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS).

Gielen F, Hours R, Emond S, Fischlechner M, Schell U, Hollfelder F. Gielen F, et al. Among authors: hollfelder f. Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):E7383-E7389. doi: 10.1073/pnas.1606927113. Epub 2016 Nov 7. Proc Natl Acad Sci U S A. 2016. PMID: 27821774 Free PMC article.

4

Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics.

Gielen F, Colin PY, Mair P, Hollfelder F. Gielen F, et al. Among authors: hollfelder f. Methods Mol Biol. 2018;1685:297-309. doi: 10.1007/978-1-4939-7366-8_18. Methods Mol Biol. 2018. PMID: 29086317

5

Single-cell activity screening in microfluidic droplets.

Neun S, Kaminski TS, Hollfelder F. Neun S, et al. Among authors: hollfelder f. Methods Enzymol. 2019;628:95-112. doi: 10.1016/bs.mie.2019.07.009. Epub 2019 Aug 8. Methods Enzymol. 2019. PMID: 31668237

6

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets.

van Loo B, Heberlein M, Mair P, Zinchenko A, Schüürmann J, Eenink BDG, Holstein JM, Dilkaute C, Jose J, Hollfelder F, Bornberg-Bauer E. van Loo B, et al. Among authors: hollfelder f. ACS Synth Biol. 2019 Dec 20;8(12):2690-2700. doi: 10.1021/acssynbio.9b00274. Epub 2019 Dec 4. ACS Synth Biol. 2019. PMID: 31738524

7

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.

Lindenburg L, Huovinen T, van de Wiel K, Herger M, Snaith MR, Hollfelder F. Lindenburg L, et al. Among authors: hollfelder f. Nucleic Acids Res. 2020 Jun 19;48(11):e63. doi: 10.1093/nar/gkaa270. Nucleic Acids Res. 2020. PMID: 32383757 Free PMC article.

8

Ultrahigh throughput screening for enzyme function in droplets.

Neun S, Zurek PJ, Kaminski TS, Hollfelder F. Neun S, et al. Among authors: hollfelder f. Methods Enzymol. 2020;643:317-343. doi: 10.1016/bs.mie.2020.06.002. Epub 2020 Jul 24. Methods Enzymol. 2020. PMID: 32896286

9

Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation.

Zurek PJ, Hours R, Schell U, Pushpanath A, Hollfelder F. Zurek PJ, et al. Among authors: hollfelder f. Lab Chip. 2021 Jan 5;21(1):163-173. doi: 10.1039/d0lc00830c. Lab Chip. 2021. PMID: 33242058

10

"NAD-display": Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry.

Lindenburg L, Hollfelder F. Lindenburg L, et al. Among authors: hollfelder f. Angew Chem Int Ed Engl. 2021 Apr 12;60(16):9015-9021. doi: 10.1002/anie.202013486. Epub 2021 Mar 8. Angew Chem Int Ed Engl. 2021. PMID: 33470025 Free PMC article.

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