Lisi GP - Search Results

1

Structural and dynamic insights into the HNH nuclease of divergent Cas9 species.

Belato HB, D'Ordine AM, Nierzwicki L, Arantes PR, Jogl G, Palermo G, Lisi GP. Belato HB, et al. Among authors: lisi gp. J Struct Biol. 2022 Mar;214(1):107814. doi: 10.1016/j.jsb.2021.107814. Epub 2021 Dec 3. J Struct Biol. 2022. PMID: 34871741 Free PMC article.

2

¹H, ¹³C, ¹⁵N backbone and side chain resonance assignment of the HNH nuclease from Streptococcus pyogenes CRISPR-Cas9.

Belato HB, East KW, Lisi GP. Belato HB, et al. Among authors: lisi gp. Biomol NMR Assign. 2019 Oct;13(2):367-370. doi: 10.1007/s12104-019-09907-9. Epub 2019 Aug 3. Biomol NMR Assign. 2019. PMID: 31377985 Free PMC article.

3

NMR and computational methods for molecular resolution of allosteric pathways in enzyme complexes.

East KW, Skeens E, Cui JY, Belato HB, Mitchell B, Hsu R, Batista VS, Palermo G, Lisi GP. East KW, et al. Among authors: lisi gp. Biophys Rev. 2020 Feb;12(1):155-174. doi: 10.1007/s12551-019-00609-z. Epub 2019 Dec 14. Biophys Rev. 2020. PMID: 31838649 Free PMC article. Review.

4

Allosteric Motions of the CRISPR-Cas9 HNH Nuclease Probed by NMR and Molecular Dynamics.

East KW, Newton JC, Morzan UN, Narkhede YB, Acharya A, Skeens E, Jogl G, Batista VS, Palermo G, Lisi GP. East KW, et al. Among authors: lisi gp. J Am Chem Soc. 2020 Jan 22;142(3):1348-1358. doi: 10.1021/jacs.9b10521. Epub 2020 Jan 9. J Am Chem Soc. 2020. PMID: 31885264 Free PMC article.

5

Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor.

Cui JY, Zhang F, Nierzwicki L, Palermo G, Linhardt RJ, Lisi GP. Cui JY, et al. Among authors: lisi gp. Biochemistry. 2020 Sep 29;59(38):3541-3553. doi: 10.1021/acs.biochem.0c00538. Epub 2020 Sep 11. Biochemistry. 2020. PMID: 32897051

6

¹H, ¹³C, ¹⁵ N backbone resonance assignment of the recognition lobe subdomain 3 (Rec3) from Streptococcus pyogenes CRISPR-Cas9.

Skeens E, East KW, Lisi GP. Skeens E, et al. Among authors: lisi gp. Biomol NMR Assign. 2021 Apr;15(1):25-28. doi: 10.1007/s12104-020-09977-0. Epub 2020 Sep 15. Biomol NMR Assign. 2021. PMID: 32935194 Free PMC article.

7

Enhanced specificity mutations perturb allosteric signaling in CRISPR-Cas9.

Nierzwicki L, East KW, Morzan UN, Arantes PR, Batista VS, Lisi GP, Palermo G. Nierzwicki L, et al. Among authors: lisi gp. Elife. 2021 Dec 15;10:e73601. doi: 10.7554/eLife.73601. Elife. 2021. PMID: 34908530 Free PMC article.

8

Structural Basis for Reduced Dynamics of Three Engineered HNH Endonuclease Lys-to-Ala Mutants for the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Associated 9 (CRISPR/Cas9) Enzyme.

Wang J, Skeens E, Arantes PR, Maschietto F, Allen B, Kyro GW, Lisi GP, Palermo G, Batista VS. Wang J, et al. Among authors: lisi gp. Biochemistry. 2022 May 3;61(9):785-794. doi: 10.1021/acs.biochem.2c00127. Epub 2022 Apr 14. Biochemistry. 2022. PMID: 35420793 Free PMC article.

9

How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM.

Wang J, Liu J, Gisriel CJ, Wu S, Maschietto F, Flesher DA, Lolis E, Lisi GP, Brudvig GW, Xiong Y, Batista VS. Wang J, et al. Among authors: lisi gp. J Struct Biol. 2022 Dec;214(4):107902. doi: 10.1016/j.jsb.2022.107902. Epub 2022 Oct 3. J Struct Biol. 2022. PMID: 36202310 Free PMC article.

10

Disruption of electrostatic contacts in the HNH nuclease from a thermophilic Cas9 rewires allosteric motions and enhances high-temperature DNA cleavage.

Belato HB, Norbrun C, Luo J, Pindi C, Sinha S, D'Ordine AM, Jogl G, Palermo G, Lisi GP. Belato HB, et al. Among authors: lisi gp. J Chem Phys. 2022 Dec 14;157(22):225103. doi: 10.1063/5.0128815. J Chem Phys. 2022. PMID: 36546784 Free PMC article.

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