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Micrococcin cysteine-to-thiazole conversion through transient interactions between a scaffolding protein and two modification enzymes.
Calvopina-Chavez DG, Bursey DM, Tseng YJ, Patil LM, Bewley KD, Bennallack PR, McPhie JM, Wagstaff KB, Daley A, Miller SM, Moody JD, Price JC, Griffitts JS. Calvopina-Chavez DG, et al. Among authors: moody jd. bioRxiv [Preprint]. 2023 Oct 23:2023.10.23.563616. doi: 10.1101/2023.10.23.563616. bioRxiv. 2023. Update in: Appl Environ Microbiol. 2024 Jun 18;90(6):e0024424. doi: 10.1128/aem.00244-24 PMID: 37961320 Free PMC article. Updated. Preprint.
Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits.
Gajjar PL, Romo MJP, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD. Gajjar PL, et al. Among authors: moody jd. bioRxiv [Preprint]. 2023 May 15:2023.05.12.540586. doi: 10.1101/2023.05.12.540586. bioRxiv. 2023. PMID: 37293010 Free PMC article. Preprint.
Micrococcin cysteine-to-thiazole conversion through transient interactions between the scaffolding protein TclI and the modification enzymes TclJ and TclN.
Calvopina-Chavez DG, Bursey DM, Tseng Y-J, Patil LM, Bewley KD, Bennallack PR, McPhie JM, Wagstaff KB, Daley A, Miller SM, Moody JD, Price JC, Griffitts JS. Calvopina-Chavez DG, et al. Among authors: moody jd. Appl Environ Microbiol. 2024 Jun 18;90(6):e0024424. doi: 10.1128/aem.00244-24. Epub 2024 May 23. Appl Environ Microbiol. 2024. PMID: 38780510 Free PMC article.
Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain.
Nawarathnage S, Tseng YJ, Soleimani S, Smith T, Romo MJP, Abiodun WO, Egbert CM, Madhusanka D, Bunn D, Woods B, Tsubaki E, Stewart C, Brown S, Doukov T, Andersen JL, Moody JD. Nawarathnage S, et al. Among authors: moody jd. bioRxiv [Preprint]. 2023 Jun 14:2023.06.14.544429. doi: 10.1101/2023.06.14.544429. bioRxiv. 2023. Update in: Structure. 2023 Dec 7;31(12):1589-1603.e6. doi: 10.1016/j.str.2023.09.001 PMID: 37398013 Free PMC article. Updated. Preprint.
Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Gajjar PL, Pedroza Romo MJ, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD. Gajjar PL, et al. Among authors: moody jd. Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):925-943. doi: 10.1107/S2059798323007246. Epub 2023 Sep 25. Acta Crystallogr D Struct Biol. 2023. PMID: 37747038 Free PMC article.
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