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The ABC toxin complex from Yersinia entomophaga can package three different cytotoxic components expressed from distinct genetic loci in an unfolded state: the structures of both shell and cargo.
Busby JN, Trevelyan S, Pegg CL, Kerr ED, Schulz BL, Chassagnon I, Landsberg MJ, Weston MK, Hurst MRH, Lott JS. Busby JN, et al. Among authors: weston mk. IUCrJ. 2024 May 1;11(Pt 3):299-308. doi: 10.1107/S2052252524001969. IUCrJ. 2024. PMID: 38512773 Free PMC article.
Warm-up before laparoscopic surgery is not essential.
Weston MK, Stephens JH, Schafer A, Hewett PJ. Weston MK, et al. ANZ J Surg. 2014 Mar;84(3):143-7. doi: 10.1111/j.1445-2197.2012.06321.x. Epub 2012 Nov 22. ANZ J Surg. 2014. PMID: 23171140 Clinical Trial.