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Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments.
Nomura K, Onda K, Murase H, Hashiya F, Ono Y, Terai G, Oka N, Asai K, Suzuki D, Takahashi N, Hiraoka H, Inagaki M, Kimura Y, Shimizu Y, Abe N, Abe H. Nomura K, et al. Among authors: murase h. RSC Chem Biol. 2024 Feb 26;5(4):360-371. doi: 10.1039/d3cb00212h. eCollection 2024 Apr 3. RSC Chem Biol. 2024. PMID: 38576723 Free PMC article.
Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.
Inagaki M, Abe N, Li Z, Nakashima Y, Acharyya S, Ogawa K, Kawaguchi D, Hiraoka H, Banno A, Meng Z, Tada M, Ishida T, Lyu P, Kokubo K, Murase H, Hashiya F, Kimura Y, Uchida S, Abe H. Inagaki M, et al. Among authors: murase h. Nat Commun. 2023 May 11;14(1):2657. doi: 10.1038/s41467-023-38244-8. Nat Commun. 2023. PMID: 37169757 Free PMC article.
Development of hydrophobic tag purifying monophosphorylated RNA for chemical synthesis of capped mRNA and enzymatic synthesis of circular mRNA.
Ototake M, Inagaki M, Kimura S, Onda K, Tada M, Kawaguchi D, Murase H, Fukuchi K, Gao Y, Kokubo K, Acharyya S, Meng Z, Ishida T, Kawasaki T, Abe N, Hashiya F, Kimura Y, Abe H. Ototake M, et al. Among authors: murase h. Nucleic Acids Res. 2024 Nov 11;52(20):12141-12157. doi: 10.1093/nar/gkae847. Nucleic Acids Res. 2024. PMID: 39414255 Free PMC article.
290 results