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Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno-associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids.
Srinivasan P, Canova CT, Sha S, Nguyen TNT, Joseph J, Sangerman J, Maloney AJ, Katsikis G, Ou RW, Hong MS, Ng J, Yuan A, Antov D, Song S, Chen W, Neufeld C, Wolfrum JM, Barone PW, Sinskey AJ, Springs SL, Braatz RD. Srinivasan P, et al. Among authors: springs sl. Biotechnol Bioeng. 2024 Dec;121(12):3694-3714. doi: 10.1002/bit.28828. Epub 2024 Aug 23. Biotechnol Bioeng. 2024. PMID: 39176568
Cellular pathways of recombinant adeno-associated virus production for gene therapy.
Sha S, Maloney AJ, Katsikis G, Nguyen TNT, Neufeld C, Wolfrum J, Barone PW, Springs SL, Manalis SR, Sinskey AJ, Braatz RD. Sha S, et al. Among authors: springs sl. Biotechnol Adv. 2021 Jul-Aug;49:107764. doi: 10.1016/j.biotechadv.2021.107764. Epub 2021 May 3. Biotechnol Adv. 2021. PMID: 33957276 Review.
Cyberbiosecurity in Advanced Manufacturing Models.
Guttieres D, Stewart S, Wolfrum J, Springs SL. Guttieres D, et al. Among authors: springs sl. Front Bioeng Biotechnol. 2019 Sep 4;7:210. doi: 10.3389/fbioe.2019.00210. eCollection 2019. Front Bioeng Biotechnol. 2019. PMID: 31552236 Free PMC article.
Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies.
Gimpel AL, Katsikis G, Sha S, Maloney AJ, Hong MS, Nguyen TNT, Wolfrum J, Springs SL, Sinskey AJ, Manalis SR, Barone PW, Braatz RD. Gimpel AL, et al. Among authors: springs sl. Mol Ther Methods Clin Dev. 2021 Feb 17;20:740-754. doi: 10.1016/j.omtm.2021.02.010. eCollection 2021 Mar 12. Mol Ther Methods Clin Dev. 2021. PMID: 33738328 Free PMC article. Review.
Mechanistic modeling explains the production dynamics of recombinant adeno-associated virus with the baculovirus expression vector system.
Destro F, Joseph J, Srinivasan P, Kanter JM, Neufeld C, Wolfrum JM, Barone PW, Springs SL, Sinskey AJ, Cecchini S, Kotin RM, Braatz RD. Destro F, et al. Among authors: springs sl. Mol Ther Methods Clin Dev. 2023 Jun 2;30:122-146. doi: 10.1016/j.omtm.2023.05.019. eCollection 2023 Sep 14. Mol Ther Methods Clin Dev. 2023. PMID: 37746245 Free PMC article.
32 results