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Directed evolution of the multicopper oxidase laccase for cell surface proximity labeling and electron microscopy.
Lee SY, Roh H, Gonzalez-Perez D, Mackey MR, Kim KY, Hoces D, McLaughlin CN, Adams SR, Nguyen K, Luginbuhl DJ, Luo L, Udeshi ND, Carr SA, Hernández-López RA, Ellisman MH, Alcalde M, Ting AY. Lee SY, et al. Among authors: gonzalez perez d. bioRxiv [Preprint]. 2024 Oct 29:2024.10.29.620861. doi: 10.1101/2024.10.29.620861. bioRxiv. 2024. PMID: 39554088 Free PMC article. Preprint.
Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics.
Álvaro-Benito M, Sainz-Polo MA, González-Pérez D, González B, Plou FJ, Fernández-Lobato M, Sanz-Aparicio J. Álvaro-Benito M, et al. J Biol Chem. 2012 Jun 1;287(23):19674-86. doi: 10.1074/jbc.M112.355503. Epub 2012 Apr 16. J Biol Chem. 2012. PMID: 22511773 Free PMC article.
Blood tolerant laccase by directed evolution.
Mate DM, Gonzalez-Perez D, Falk M, Kittl R, Pita M, De Lacey AL, Ludwig R, Shleev S, Alcalde M. Mate DM, et al. Chem Biol. 2013 Feb 21;20(2):223-31. doi: 10.1016/j.chembiol.2013.01.001. Chem Biol. 2013. PMID: 23438751
Directed evolution of unspecific peroxygenase from Agrocybe aegerita.
Molina-Espeja P, Garcia-Ruiz E, Gonzalez-Perez D, Ullrich R, Hofrichter M, Alcalde M. Molina-Espeja P, et al. Appl Environ Microbiol. 2014 Jun;80(11):3496-507. doi: 10.1128/AEM.00490-14. Epub 2014 Mar 28. Appl Environ Microbiol. 2014. PMID: 24682297 Free PMC article.
Bioelectrochemical oxidation of water.
Pita M, Mate DM, Gonzalez-Perez D, Shleev S, Fernandez VM, Alcalde M, De Lacey AL. Pita M, et al. J Am Chem Soc. 2014 Apr 23;136(16):5892-5. doi: 10.1021/ja502044j. Epub 2014 Apr 11. J Am Chem Soc. 2014. PMID: 24725275
32 results