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A consensus platform for antibody characterization.
Ayoubi R, Ryan J, Gonzalez Bolivar S, Alende C, Ruiz Moleon V, Fotouhi M, Alqazzaz M, Southern K, Alshafie W, Baker MR, Ball AR Jr, Callahan D, Cooper JA, Crosby K, Harvey KJ, Houston DW, Kumaran R, Rego M, Schofield C, Wu H, Biddle MS, Brown CM, Kahn RA, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. Ayoubi R, et al. Among authors: laflamme c. Nat Protoc. 2024 Dec 17. doi: 10.1038/s41596-024-01095-8. Online ahead of print. Nat Protoc. 2024. PMID: 39690206 Review.
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.
Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Moleon VR, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown CM, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. Ayoubi R, et al. Among authors: laflamme c. bioRxiv [Preprint]. 2023 Aug 17:2023.06.01.543292. doi: 10.1101/2023.06.01.543292. bioRxiv. 2023. Update in: Elife. 2023 Nov 23;12:RP91645. doi: 10.7554/eLife.91645 PMID: 37398479 Free PMC article. Updated. Preprint.
The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescence.
Ayoubi R, Fotouhi M, Southern K, Bhajiawala R, Fanti R, Prinos P, McPherson PS, Laflamme C; NeuroSGC/YCharOS/EDDU collaborative group; ABIF Consortium. Ayoubi R, et al. Among authors: laflamme c. F1000Res. 2023 Mar 21;12:308. doi: 10.12688/f1000research.131333.1. eCollection 2023. F1000Res. 2023. PMID: 37545650 Free PMC article.
The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence.
Alshafie W, Fotouhi M, Ayoubi R, Shlaifer I, Southern K, McPherson PS, Laflamme C; NeuroSGC/YCharOS/EDDU collaborative group. Alshafie W, et al. Among authors: laflamme c. F1000Res. 2023 Jul 25;12:884. doi: 10.12688/f1000research.139755.1. eCollection 2023. F1000Res. 2023. PMID: 37635943 Free PMC article.
119 results