Wetzel R - Search Results

1

Expression in Escherichia coli of a chemically synthesized gene for a "mini-C" analog of human proinsulin.

Wetzel R, Kleid DG, Crea R, Heyneker HL, Yansura DG, Hirose T, Kraszewski A, Riggs AD, Itakura K, Goeddel DV. Wetzel R, et al. Gene. 1981 Dec;16(1-3):63-71. doi: 10.1016/0378-1119(81)90061-5. Gene. 1981. PMID: 7044895

2

Non-toxic expression in Escherichia coli of a plasmid-encoded gene for phage T4 lysozyme.

Perry LJ, Heyneker HL, Wetzel R. Perry LJ, et al. Among authors: wetzel r. Gene. 1985;38(1-3):259-64. doi: 10.1016/0378-1119(85)90226-4. Gene. 1985. PMID: 3905515

3

Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene.

Wetzel R, Heyneker HL, Goeddel DV, Jhurani P, Shapiro J, Crea R, Low TL, McClure JE, Thurman GB, Goldstein AL. Wetzel R, et al. Biochemistry. 1980 Dec 23;19(26):6096-104. doi: 10.1021/bi00567a023. Biochemistry. 1980. PMID: 7008828

4

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

Cabilly S, Riggs AD, Pande H, Shively JE, Holmes WE, Rey M, Perry LJ, Wetzel R, Heyneker HL. Cabilly S, et al. Among authors: wetzel r. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3273-7. doi: 10.1073/pnas.81.11.3273. Proc Natl Acad Sci U S A. 1984. PMID: 6374653 Free PMC article.

5

Properties of a human alpha-interferon purified from E. coli extracts.

Wetzel R, Perry LJ, Estell DA, Lin N, Levine HL, Slinker B, Fields F, Ross MJ, Shively J. Wetzel R, et al. J Interferon Res. 1981;1(3):381-90. doi: 10.1089/jir.1981.1.381. J Interferon Res. 1981. PMID: 6180070

6

Disulfide bonds and thermal stability in T4 lysozyme.

Wetzel R, Perry LJ, Baase WA, Becktel WJ. Wetzel R, et al. Proc Natl Acad Sci U S A. 1988 Jan;85(2):401-5. doi: 10.1073/pnas.85.2.401. Proc Natl Acad Sci U S A. 1988. PMID: 3277175 Free PMC article.

7

The role of cysteine oxidation in the thermal inactivation of T4 lysozyme.

Perry LJ, Wetzel R. Perry LJ, et al. Among authors: wetzel r. Protein Eng. 1987 Feb-Mar;1(2):101-5. doi: 10.1093/protein/1.2.101. Protein Eng. 1987. PMID: 3507692

8

Unpaired cysteine-54 interferes with the ability of an engineered disulfide to stabilize T4 lysozyme.

Perry LJ, Wetzel R. Perry LJ, et al. Among authors: wetzel r. Biochemistry. 1986 Feb 11;25(3):733-9. doi: 10.1021/bi00351a034. Biochemistry. 1986. PMID: 3513834

We have introduced an intramolecular disulfide bond into T4 lysozyme and have shown this molecule to be significantly more stable than the wild-type molecule to irreversible thermal inactivation [Perry, L.J., & Wetzel, R. (1984) Science (Washington, D.C.) 226, 5 …

We have introduced an intramolecular disulfide bond into T4 lysozyme and have shown this molecule to be significantly more stable than the w …

9

Disulfide bond engineered into T4 lysozyme: stabilization of the protein toward thermal inactivation.

Perry LJ, Wetzel R. Perry LJ, et al. Among authors: wetzel r. Science. 1984 Nov 2;226(4674):555-7. doi: 10.1126/science.6387910. Science. 1984. PMID: 6387910

10

Effect of an engineered disulfide bond on the folding of T4 lysozyme at low temperatures.

Anderson WD, Fink AL, Perry LJ, Wetzel R. Anderson WD, et al. Among authors: wetzel r. Biochemistry. 1990 Apr 3;29(13):3331-7. doi: 10.1021/bi00465a026. Biochemistry. 1990. PMID: 2334694

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