High activity and stability of hammerhead ribozymes containing 2'-modified pyrimidine nucleosides and phosphorothioates.
Heidenreich O, Benseler F, Fahrenholz A, Eckstein F.
Heidenreich O, et al. Among authors: eckstein f.
J Biol Chem. 1994 Jan 21;269(3):2131-8.
J Biol Chem. 1994.
PMID: 8294467
Free article.
Previous studies had shown that substitution of all pyrimidine nucleosides by their 2'-fluoro analogs led to an 8-fold decrease in catalytic efficiency in the cleavage reaction compared to the unmodified ribozyme (Heidenreich, O., and Eckstein, F. (1992) J. Biol. Ch …
Previous studies had shown that substitution of all pyrimidine nucleosides by their 2'-fluoro analogs led to an 8-fold decrease in catalytic …