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Purified human vitamin D receptor overexpressed in E. coli and baculovirus systems does not bind 1,25-dihydroxyvitamin D3 hormone efficiently unless supplemented with a rat liver nuclear extract.
Nakajima S, Hsieh JC, MacDonald PN, Haussler CA, Galligan MA, Jurutka PW, Haussler MR. Nakajima S, et al. Among authors: galligan ma. Biochem Biophys Res Commun. 1993 Dec 15;197(2):478-85. doi: 10.1006/bbrc.1993.2504. Biochem Biophys Res Commun. 1993. PMID: 8267583
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